Impacts of chloramine-T treatment on antioxidant enzyme activities and genotoxicity in rainbow trout, Oncorhynchus mykiss (Walbaum)

Boran H., ALTINOK İ.

JOURNAL OF FISH DISEASES, vol.37, no.5, pp.431-441, 2014 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 37 Issue: 5
  • Publication Date: 2014
  • Doi Number: 10.1111/jfd.12122
  • Journal Name: JOURNAL OF FISH DISEASES
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.431-441
  • Keywords: rainbow trout, comet assay, chloramine-T, enzyme activity, genotoxicity, GLUTATHIONE S-TRANSFERASES, COMET ASSAY, EXPOSURE, FISH, PESTICIDES, CATFISH, WORKERS, PLASMA, GROWTH, BLOOD
  • Karadeniz Technical University Affiliated: Yes

Abstract

Juvenile rainbow trout Oncorhynchus mykiss (Walbaum) were exposed to therapeutic, and higher concentrations of chloramine-T (Cl-T) to assess the effects of this chemical on the antioxidant enzyme system and genetic structure. Red blood cells acetylcholinesterase, increment -aminolevulinic acid dehydratase, paraoxonase and liver glutathione S-transferase activity were increased at 10 and 20mg L-1 Cl-T-exposed fish, while they were decreased at 30mg L-1 Cl-T-exposed fish. On the other hand, liver catalase activity and liver protein levels increased at 10mg L-1 and decreased at 20 and 30mg L-1 concentrations of Cl-T. Liver super-oxide dismutase activity decreased at 10mg L-1 and 20mg L-1 Cl-T and increased at 30mg L-1 of Cl-T. Compared to control, comet assay indicated that Cl-T did not cause significant DNA damage to red blood cells of the fish. Results indicate that 10 or 20mg L-1 Cl-T can be safely used to prevent or treat external parasitic and bacterial infection of rainbow trout.