Cellular responses of spirlin Alburnoides bipunctatus spermatozoa exposed to Bisphenol A: Biochemical response and sperm quality alterations evaluation


KOCABAŞ M., KUTLUYER F., SAHİLLİ Y. Ç. , AKSU Ö.

HUMAN AND ECOLOGICAL RISK ASSESSMENT, 2020 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume:
  • Publication Date: 2020
  • Doi Number: 10.1080/10807039.2020.1715784
  • Journal Name: HUMAN AND ECOLOGICAL RISK ASSESSMENT
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, PASCAL, Aerospace Database, Agricultural & Environmental Science Database, Aqualine, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, CAB Abstracts, Chemical Abstracts Core, Communication Abstracts, Environment Index, Food Science & Technology Abstracts, Greenfile, Metadex, Pollution Abstracts, Public Affairs Index, Veterinary Science Database, Civil Engineering Abstracts
  • Keywords: Alburnoides bipunctatus, spirlin, Bisphenol A, antioxidant enzymes, spermatozoa, toxicity, CARP CYPRINUS-CARPIO, OXIDATIVE STRESS INDEXES, DNA INTEGRITY, ANTIOXIDANT RESPONSES, ENDOCRINE DISRUPTERS, WASTE-WATER, BROWN TROUT, MOTILITY, TOXICITY, URINARY
  • Karadeniz Technical University Affiliated: Yes

Abstract

Plastics are one of the global environmental problems owing to their highest production. Bisphenol A (BPA) is used in the manufacturing of plastics including polycarbonate, polyvinyl chloride and resins. In particular, aquatic ecosystems are adversely affected by the occurrence of micropollutants. In addition, BPA as a typical endocrine-disrupting chemical (EDC) seriously influences androgenic activity and reproduction of aquatic organisms, particularly fish as non-target organism. In toxicological assays, sperm cells can be used as a potential tool for reliable and practical assessment. For these reasons, sperm cells were exposed to lower concentrations (0 mu g/L, 0.5 mu g/L, 1.25 mu g/L, 2.5 mu g/L and 5 mu g/L) of BPA and the study focused on BPA-induced oxidative stress. The enzymatic [glutathione peroxidase (GSH-Px), catalase (CAT) and superoxide dismutase (SOD)] activities and lipid peroxidation (MDA) were analyzed in spermatozoa. The study findings showed that the activities of SOD and GSH-Px were significantly decreased by BPA but lipid peroxidation (MDA) and CAT acticity increased. Notably, the percentage and duration of motile sperm cells decreased. Collectively, spermatozoa clearly showed the sensitivity even at extremely low concentrations. This is first report to assess the impact of BPA on sperm cells of spirlin Alburnoides bipunctatus.