Akademik Veri Yönetim Sistemi
3rd International Conference on Engineering and Natural Science (ICENS), Budapest, Macaristan, 3 - 07 Mayıs 2017
Streptomycetes are medically and biotechnologically important bacteria because they are the producers of a wide variety of bioactive compounds (anticancer agents, immunosuppressant drugs, herbicides etc.), including 75 % of the commercialized antibiotics. To the best of our knowledge, effect of ATP dependent Lon proteases, which are one of the most important enzyme in protein degradation, on secondary metabolite production is not known and is an interesting subject waiting to be clarified. In the present study, we constructed a recombinant strain by inserting one extra copy of lon gene into the Streptomyces coelicolor A(3)2 genome. For this, lon gene was amplified from S. coelicolor with its own promotor and transcriptional terminator regions by PCR. After verification by DNA sequencing, it was cloned into shuttle vector pRA. Then S. coelicolor A(3)2 protoplasts were transformed with this recombinant vector. Actinorhodin and undecylprodigiosin production by recombinant S. coelicolor grown in R2YE medium for 120 hour was determined according to Kieser et al. (2000). Preliminary results for the effect of extra copy of lon gene on the production of antibiotics by S. coelicolor were obtained in this study.