Biological and Phytochemical Investigations of <i>Alchemilla daghestanica</i> Juz. and <i>Alchemilla minusculiflora</i> Buser: A Focus on Anti-Inflammatory and Skin-Regenerative Properties

Ortahisar M., Cigsar T., SEYHAN G., AKKAYA D., Uzuner S., BARUT B., ...More

CHEMISTRY & BIODIVERSITY, vol.22, no.12, 2025 (SCI-Expanded, Scopus) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 22 Issue: 12
  • Publication Date: 2025
  • Doi Number: 10.1002/cbdv.202500539
  • Journal Name: CHEMISTRY & BIODIVERSITY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Agricultural & Environmental Science Database, Aquatic Science & Fisheries Abstracts (ASFA), CAB Abstracts, Chemical Abstracts Core, EMBASE, MEDLINE, Veterinary Science Database
  • Keywords: Alchemilla, ethnopharmacology, healthcare, natural product, wound healing
  • Karadeniz Technical University Affiliated: Yes

Abstract

The biological activities and phytochemical composition of Alchemilla daghestanica and Alchemilla minusculiflora were investigated for the first time. Methanol extracts from the aerial and root parts of both species were assessed. The total phenolic content was highest in the root extracts. A. daghestanica root (Alda root) and A. minusculiflora root (Almin root) exhibited by 91.58% radical scavenging activity at 200 mu g/mL, comparable to gallic acid (91.98%). Alda root inhibited COX-1 by 85.79% and COX-2 by 91.89% at 200 mu g/mL, while Almin root inhibited COX-1 by 83.99% and COX-2 by 91.82%. Elastase inhibition was highest in Almin root (IC50 = 40.35 mu g/mL). No significant collagenase inhibition was found. Wound-healing assays demonstrated that root extracts exhibited the most rapid closure at 250 mu g/mL after 24 h. Key bioactive compounds, identified by LC-MS/MS, were catechin and ellagic acid, which may contribute to the observed biological effects.