© 2018 Turkish Biochemistry Society. All rights reserved.Objective: The purpose of this study was therefore to investigate various physicochemical properties, phenolic composition and antioxidant capacities of the oak honey, large quantities of which are produced in the Thrace region of Northwest Turkey. Methods: Hunter color values (Lab), optical rotation, electrical conductivity, moisture and ash were measured as physicochemical determinants. Sugar, proline content, mineral, phenolic acids, total phenolic flavonoids contents were evaluated as chemical parameters. Ferric reducing antioxidant power (FRAP) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging were used as antioxidant determinants. Results: The results determined that dark-colored honeys (mean 24.95 ± 8.35 L) have proline levels (649.82 ± 203.90 mg/kg), total phenolic content (TPC) (67.29 ± 13.10 mg GAE/100 g) and total flavonoid content (TFC) (10.14 ± 4.78 mg QE/100 g), a high ratio of fructose/ glucose (1.65 ± 0.18). Rutin (11.14 ± 8.50 μg/g), p-coumaric acid (6.97 ± 5.80 μg/g) and protocatechuic acid (6.12 ± 4.39 μg/g) were the major phenolic components, and quercetin was also detected in varying amounts in all samples. Potassium (2523 ± 0.28 mg/kg), calcium (63.40 ± 3.20 mg/ kg) and magnesium (38.88 ± 0.65 mg/kg) were the major minerals in the honeys. Conclusion: Our results showed that physicochemical properties, phenolic compounds, antioxidant capacity, sugar and mineral contents successfully distinguish the geographical origins of oak honey. These findings will contribute to a more accurate evaluation of oak honey in the literature.